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Transport of malate and chloride into barley mesophyll vacuoles Different carriers are involved
Author(s) -
Martinoia Enrico,
Vogt Esther,
Amrhein Nikolaus
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80648-3
Subject(s) - vacuole , chloride , chemistry , phosphate , biochemistry , malic acid , citric acid , cytoplasm , organic chemistry
Transport of malate and chloride across the tonoplast of barley mesophyll vacuoles has been compared. Whereas malate inhibited chloride uptake, 1,2,3‐benzenetricarboxylic acid, a potent inhibitor of malate uptake which is assumed not to cross the tonoplast, was ineffective. In contrast to chloride uptake, malate uptake was inactivated by pyridoxal phosphate and phenylglyoxal. The malate transport system could be protected against pyridoxal phosphate when a substrate such as 1,2,3‐benzenetricarboxylic acid was present in the preincubation medium. It is concluded that the transfer of malate and chloride across the tonoplast is mediated by different systems.