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Functional modes of retinoic acid in mouse osteoblastic clone MC3T3‐E1, proved as a target cell for retinoic acid
Author(s) -
Nakayama Yasuhiro,
Takahashi Kojiro,
Nqji Sumihare,
Muto Kohki,
Nishijima Katsumi,
Taniguchi Shigehiko
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80644-x
Subject(s) - retinoic acid , clone (java method) , northern blot , retinoic acid inducible orphan g protein coupled receptor , alkaline phosphatase , tretinoin , microbiology and biotechnology , retinoic acid receptor , western blot , chemistry , biology , cell , cell growth , receptor , gene , gene expression , biochemistry , enzyme
Mouse osteoblastic clone MC3T3‐E1 was proved as a target cell for retinoic acid (RA) in bone tissues through the demonstration of RA‐receptor gene expression by the northern blot analysis. The effect of RA on the cell growth of MC3T3‐E1 was repressive for both subconfluent and confluent growth, whereas RA enhancement of alkaline phosphatase expression was observed at the confluent stage. This implies that RA is a regulatory factor leading osteogenesis of the cells after the confluent stage. RA exhibited simultaneously the stage‐dependent effects on EGF‐dependent mitogenesis: promotive at the subconfluent, but repressive at the confluent stage.