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Members of the α‐amylase inhibitors family from wheat endosperm are major allergens associated with baker's asthma
Author(s) -
Gómez Luis,
Martin Elena,
Hernández Dolores,
Sánchez-Monge Rosa,
Barber Domingo,
del Pozo Victoria,
de Andres Belén,
Armentia Alicia,
Lahoz Carlos,
Salced Gabriel,
Palomino Filar
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80642-v
Subject(s) - endosperm , immunoglobulin e , fractionation , amylase , chemistry , biochemistry , enzyme , allergen , biology , immunology , allergy , chromatography , antibody
We have identified the major antigens or IgE binding components from wheat flour. Thirty‐five sera from patients with baker's asthma were used to analyze the reaction with wheat salt‐soluble proteins. We found a 15 kDa SDS‐PAGE band which reacted with all sera tested. Purified members of the α‐amylase inhibitor family, which are the main components of the 15 kDa band, were recognized by specific IgE when tested with a pool of reactive sera. Immunodetection after two‐dimensional electrophoretic fractionation of crude inhibitor preparations from wheat endosperms also detected several inhibitor subunits as major low‐molecular‐weight allergens.