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Enzymatic synthesis of dihydrosirohydrochlorin (precorrin‐2) and of a novel pyrrocorphin by uroporphyrinogen III methylase
Author(s) -
Warren Martin J.,
Stolowich Neal J.,
Santander Patricio J.,
Roessner Charles A.,
Sowa Blair A.,
Scott A.Ian
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80640-5
Subject(s) - methylation , enzyme , chemistry , biochemistry , gene , recombinant dna , stereochemistry , plasmid , microbiology and biotechnology , biology
Uroporphyrinogen III methylase was purified from a recombinant hem B − strain of E. coli harbouring a plasmid containing the cys G gene. N‐terminal analysis of this purified protein gave an amino acid sequence corresponding to that predicted from the genetic code. From the u.v./visible spectrum of the reaction catalysed by this SAM dependent methylase it was possible to observe the sequential appearance of the chromophores of a dipyrrocorphin and subsequently of a pyrrocorphin. Confirmation of this transformation was obtained from 13 C‐NMR studies when it was demonstrated, for the first time directly, that uroporphyrinogen is initially converted into dihydrosirohydrochlorin (precorrin‐2) and then, by further methylation, into a novel trimethylpyrrocorphin.