Premium
Monoclonal antibodies against the presynaptic calcium channel antagonist ω‐conotoxin GVI A from cone snail poison
Author(s) -
Tombaccini D.,
Adeyemo O.M.,
Pollard H.B.,
Feuerstein G.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80639-z
Subject(s) - conotoxin , monoclonal antibody , conus , toxin , chemistry , snail , microbiology and biotechnology , antibody , biology , biochemistry , peptide , anatomy , immunology , ecology
Monoclonal antibodies have been prepared against co‐conotoxin GVI A, a peptide isolated from marine snails of the genus Conus (Conus geographus and Conus magus ). This toxin is a blocker of select presynaptic Ca 2+ channels in the central nervous system. Antigenic ω‐conotoxin GVI A was synthesized as a covalent conjugate with bovine serum albumin and injected s.c. An ELISA assay combined with a competitive inhibition assay was used to select and characterize monoclonal antibodies able to recognize and bind the free toxin. Several of the antibodies were found to block ω‐conotoxin GVI A inhibition of 45 Ca transport into rat brain synaptosomes and to block ω‐conotoxin GVI A binding to membranes from the same preparation. The antibodies recognize native, synthetic toxin, and are useful for analysis of toxin in biological fluids.