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Mitogen‐induced oscillations of membrane potential and Ca 2+ in human fibroblasts
Author(s) -
Peres Antonio,
Giovannardi Stefano
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80630-2
Subject(s) - chemistry , biophysics , membrane potential , mitogen activated protein kinase , microbiology and biotechnology , biochemistry , biology , phosphorylation
Using the whole‐cell technique, we have measured recurring hyperpolarizations induced by fetal calf serum and bradykinin in human fibroblasts. By coupling fura‐2 microfluorimetry to electrophysiology, we have also measured directly cytosolic Ca 2+ and found that Ca 2+ oscillations occur in synchrony with membrane currents. Mitogen stimulation of cells in which intracellular K + had been replaced with Cs + resulted in the abolishment of the outward current. We conclude then that the mitogen‐induced recurring hyperpolarizations in human fibroblasts are due to the opening of Ca 2+ ‐activated K + channels.