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Large scale preparation of homogeneous bacteriorhodopsin
Author(s) -
Lorber Bernard,
DeLucas Larry J.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80625-s
Subject(s) - bacteriorhodopsin , homogeneous , halobacterium , homogeneity (statistics) , halobacterium salinarum , chemistry , halobacteriaceae , biophysics , membrane , biochemistry , chemical engineering , biology , physics , mathematics , thermodynamics , statistics , engineering
Homogeneous bacteriorhodopsin was obtained preparatively (100 mg batches) from purple membrane of Halobacterium halobium cells. The homogeneity of the protein was considerably affected by variations in the growth conditions of the bacteria. Fully maturated bacteriorhodopsin having a blocked N‐terminus and a homogeneous C‐terminus, was reproducibly obtained when cells were grown in a sufficiently aerated medium.