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Are there isoenzymes of cytochrome c oxidase in Paracoccus denitrificans ?
Author(s) -
Raitio Mirja,
Pispa Johanna M.,
Metso Tuula,
Saraste Matti
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80609-m
Subject(s) - paracoccus denitrificans , cytochrome c oxidase , cytochrome b , biology , cytochrome , cytochrome c , protein subunit , microbiology and biotechnology , gene , mutant , biochemistry , cytochrome c1 , enzyme , mitochondrion , mitochondrial dna
We have used a gene replacement strategy to delete the previously isolated gene [(1987) EMBO J. 6, 2825‐2833] for the cytochrome c oxidase subunit I from Paracoccus denitrificans . The resulting mutant was still able to synthesize active cytochrome c oxidase. This led us to look for another locus which could completely suppress the mutation. In this study we report the isolation of a second gene encoding subunit I. An open reading frame coding for cytochrome c 550 was found upstream from this gene. We suggest that there are isoenzymes of cytochrome c oxidase (cytochrome aa 3 ) in this bacterium.