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The first observation of O 2 − generation in in situ lungs of rats treated with drugs to induce experimental acute respiratory distress syndrome
Author(s) -
Takahashi Atsushi,
Nakano Minoru,
Mashiko Shinro,
Inaba Humio
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80594-9
Subject(s) - chemiluminescence , superoxide dismutase , chemistry , in situ , phorbol , luminescence , superoxide , lung , respiratory distress , luminescent measurements , acute respiratory distress , reactive oxygen species , medicine , endocrinology , nuclear chemistry , biochemistry , oxidative stress , chromatography , anesthesia , enzyme , materials science , organic chemistry , optoelectronics , protein kinase c
To investigate O 2 − generation in in situ lungs of rats treated with drugs to induce experimental acute respiratory distress syndrome, phorbol myristate acetate (PMA) or endotoxin were injected into rats, who had been continuously infused with 2(methyl‐6‐[ p ‐methoxyphenyl]‐3,7‐dihydro‐imidazo[l,2‐α]pyrazin‐3‐one (MCLA) and the chemiluminescence in in situ lung was detected by a sensitive photon counter. In PMA‐treated rats, two phases of chemiluminescence over non‐specific chemiluminescence were observed. The first phase luminescence was sensitive to Cu‐Zn superoxide dismutase, while the second phase chemiluminescence was less sensitive to Cu‐Zn Superoxide dismutase. Similar chemiluminescence was detected in the rats treated with endotoxin instead of PMA, but not in neutropenic rats.