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De novo protein synthesis is essential to human interferon γ gene expression by the stimulation with polyI:polyC
Author(s) -
Tamura-Nishimura Motoko,
Sasakawa Shigeru
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80587-9
Subject(s) - cycloheximide , protein biosynthesis , microbiology and biotechnology , messenger rna , biology , gene expression , interferon , transcription (linguistics) , protein kinase c , rna , gene , kinase , biochemistry , virology , linguistics , philosophy
Transcription of human interferon (IFN) γ gene is induced in human peripheral lymphocyte nylon‐nonadherent cells (NNA cells) by double strand RNA poly I:poly C [(1985) J. Interferon Res. 5, 77‐84]. In this report, the necessity of de novo protein synthesis in an early stage of IFN γ gene expression is described. For induction of IFN γ gene expression, only initial 4 h treatment of poly I:poly C to NNA cells is sufficient. Addition of inhibitor of protein synthesis, cycloheximide (CHX), at an early stage of induction periods (0–4 h) inhibits the IFN γ induction by poly I:poly C. Cell free translation assay using RNAs isolated from NNA cells which are induced by poly I:poly C in the presence of CHX reveals that in these RNAs, IFN γ mRNA does not exist. These results demonstrate that CHX inhibits de novo synthesis of a certain protein (or proteins) and for lack of the protein(s), IFN γ mRNA cannot be transcribed. The evidence is also described in this report which suggests that the essential protein(s) might be that (those) involved in protein kinase C (pkC) activation.