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Cytotoxic activity of tumor necrosis factor is inhibited by amiloride derivatives without involvement of the Na + /H + antiporter
Author(s) -
Vanhaesebroeck B.,
Cragoe E.J.,
Pouysségur J.,
Beyaert R.,
Van Roy F.,
Fiers W.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80581-3
Subject(s) - amiloride , antiporter , cytotoxicity , tumor necrosis factor alpha , sodium–hydrogen antiporter , cytotoxic t cell , lysis , chemistry , microbiology and biotechnology , pharmacology , biochemistry , sodium , biology , endocrinology , membrane , in vitro , organic chemistry
Cytotoxicity of tumor necrosis factor (TNF) on L929s cells was efficiently blocked by several amiloride analogs but not by amiloride itself. This protection did not require RNA or protein synthesis. Na + /H + antiporter‐negative L‐M(TK − ) cells (LAP) could be killed by TNF, showing that the Na + /H + exchanger is not required for TNF‐cytotoxicity. Similar protection against TNF‐mediated cell lysis by amiloride derivatives was found for LAP and L929s cells, excluding a blockade of the Na + /H + antiporter as the cause of the protection against TNF by these agents.