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Alternative splicing gives rise to two forms of the p68 Ca 2+ ‐binding protein
Author(s) -
Moss Stephen E.,
Crumpton Michael J.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80576-5
Subject(s) - immunoprecipitation , alternative splicing , rna splicing , splice , cloning (programming) , antiserum , sequence (biology) , consensus sequence , peptide sequence , blot , microbiology and biotechnology , nucleic acid sequence , biology , chemistry , gene , genetics , messenger rna , rna , antibody , computer science , programming language
The p68 Ca 2+ and phospholipid binding protein of the lipocortin/calpactin family appears to exist as two forms. These may be resolved into a closely‐spaced polypeptide doublet by SDS‐PAGE. The cloning and sequencing of p68 revealed an apparent 18 nucleotide alternative splice sequence, which could account for this observation. We show here that an antiserum directed against a synthetic peptide corresponding to the region containing the splice sequence, recognises only the upper band of the p68 doublet by both immunoprecipitation and Western blotting., These results are consistent with alternative splicing being responsible for the generation of the two forms of p68.