Premium
Rapid up‐ and down‐regulation of hexokinase II in rat skeletal muscle in response to altered contractile activity
Author(s) -
Weber Franz E.,
Pette Dirk
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80574-3
Subject(s) - hexokinase , stimulation , methionine , medicine , in vivo , endocrinology , skeletal muscle , chemistry , enzyme assay , enzyme , biochemistry , biology , glycolysis , amino acid , microbiology and biotechnology
An up to 14‐fold increase in total hexokinase activity induced by low‐frequency stimulation in rat fast‐twitch muscle was followed by a rapid decay in enzyme activity after cessation of stimulation. In vivo labeling revealed that these alterations were related to rapid changes in [ 35 S]methionine incorporation into hexokinase II. A recovery period of 15 h after cessation of stimulation was sufficient to normalize the approximately 30‐fold elevated [ 35 S]methionine incorporation.