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Acetyl‐CoA carboxylase: a rapid novel assay procedure used in conjunction with the preparation of enzyme from maize leaves
Author(s) -
Howard Joy L.,
Ridley Stuart M.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80567-3
Subject(s) - acetyl coa carboxylase , enzyme , pyruvate carboxylase , chemistry , biochemistry , chromatography , enzyme assay
Acetyl‐CoA carboxylase is known to be the site of action of two groups of grass‐selective herbicides: the aryloxyphenoxypropionates and the cyclo‐hexanediones. This enzyme was extracted from maize leaves, and a novel, highly sensitive assay system developed, utilising measurement of phosphate to estimate enzyme activity. This assay system was verified by using inhibition by fluazifop and avidin to compare it with the more widely used 14 C assay. K m values for acetyl‐CoA were measured, and inhibition kinetics investigated.