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Mapping the copper ligands of Cu,Zn superoxide dismutase by nuclear overhauser enhancement of the isotropically shifted 1 H‐NMR lines of the Cu,Co derivative
Author(s) -
Paci Maurizio,
Desideri Alessandro,
Sete Marco,
Falconi Mattia,
Rotilio Giuseppe
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80560-6
Subject(s) - copper , chemistry , nuclear overhauser effect , hyperfine structure , superoxide dismutase , imidazole , nuclear magnetic resonance , crystallography , mössbauer spectroscopy , two dimensional nuclear magnetic resonance spectroscopy , proton nmr , nuclear magnetic resonance spectroscopy , stereochemistry , enzyme , atomic physics , physics , biochemistry , organic chemistry
Transient nuclear Overhauser effect (NOE) experiments were made with bovine Cu,Co Superoxide dismutase on the hyperfine shifted resonances of protons of the imidazole groups bound to the Cu.Zn cluster of the native enzyme. Assignments of lines based on the observed magnetization transfers were satisfactorily obtained only by altering the arrangement of the ligands around the catalytically active copper shown by X‐ray crystallography.