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Cloning and sequence analysis of a cDNA encoding a precursor for rat C‐type natriuretic peptide (CNP)
Author(s) -
Kojima Masayasu,
Minamino Naoto,
Kangawa Kenji,
Matsuo Hisayuki
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80544-s
Subject(s) - complementary dna , signal peptide , npr2 , cdna library , biology , microbiology and biotechnology , peptide sequence , cloning (programming) , natriuretic peptide , peptide , sequence analysis , coding region , messenger rna , northern blot , npr1 , molecular cloning , gene , biochemistry , medicine , heart failure , computer science , programming language
Recent identification of a C‐type natriuretic peptide (CNP) in porcine brain strongly suggested that a third member of the natriuretic peptide family still remains to be identified in other species of mammals. A cDNA encoding a precursor for rat CNP was cloned from a rat brain cDNA library and sequenced. The precursor was a 126‐residue peptide, carrying a 23‐residue signal sequence at the N‐terminus and the known porcine CNP‐53 sequence at the C‐terminus. By RNA blot analysis, rat CNP mRNA was found to be expressed exclusively in the brain, implying that CNP may function in the central nervous system as a neuropeptide.