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NADPH‐dependent flavoenzymes catalyze one electron reduction of metal ions and molecular oxygen and generate hydroxyl radicals
Author(s) -
Shi Xianglin,
Dalal N.S.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80539-u
Subject(s) - chemistry , radical , one electron reduction , oxidoreductase , photochemistry , ferredoxin , metal ions in aqueous solution , redox , hydroxyl radical , metal , nad+ kinase , reactive oxygen species , enzyme , inorganic chemistry , organic chemistry , biochemistry , electrochemistry , electrode
This study reports a new property of the important NAD(P)H‐dependent flavoenzymes, glutathione reductase, lipoyl dehydrogenase and ferredoxin‐NADP + oxidoreductase, that can catalyze a one electron reduction of metal ions such as chromium(VI) and vanadium(V). During the enzymatic reduction process, molecular oxygen is reduced to H 2 O 2 , which reacts with the reduced metal complexes to generate hydroxyl radicals. Since the hydroxyl radicals have been suggested to play an important role in Cr(VI) toxicity, this study provides a basis for a recent observation that Cr(VI) mutagenesis is strongly oxygen dependent. These results also point to an enzymatic pathway for the metabolism of some metal ions and concomitant generation of hydroxyl radicals.