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Agonist‐induced down‐regulation of m1 muscarinic receptors and reduction of their mRNA level in a transfected cell line
Author(s) -
Wang Shou-Zhen,
Hu Jingru,
Long Rochelle M.,
Pou Wanida S.,
Forray Carlos,
El-Fakahany Esam E.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80538-t
Subject(s) - muscarinic acetylcholine receptor , muscarinic acetylcholine receptor m2 , muscarinic acetylcholine receptor m1 , agonist , muscarinic acetylcholine receptor m3 , chinese hamster ovary cell , microbiology and biotechnology , receptor , endocrinology , muscarinic antagonist , chemistry , medicine , enzyme linked receptor , northern blot , muscarinic acetylcholine receptor m5 , biology , messenger rna , biochemistry , gene
Agonist‐induced reduction in both the number of ml muscarinic receptors and the mRNA coding for the receptor protein was investigated in Chinese hamster ovary (CHO) cells which were transfected with the ml muscarinic receptor gene. Receptor concentration was measured by the specific binding of the muscarinic ligand, [ 3 H]quinuclidinyl benzilate ([ 3 H]QNB), and Northern blot hybridization analysis was used to evaluate the levels of receptor mRNA. Incubation of cells with 1 mM of the muscarinic agonist, carbamylcholine (CBC), for 24 h decreased receptor density and mRNA levels in cells by 65% and 73%, respectively. These results indicate that agonist‐induced down‐regulation of ml muscarinic receptors might be due to, at least in part, a decrease in receptor synthesis resulting from a reduction in the steady‐state level of their mRNA.