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Activation of red cell Ca 2+ ‐activated K + channel by Ca 2+ involves a temperature‐dependent step
Author(s) -
Varečka Ĺudovít,
Peterajová Elena
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80534-p
Subject(s) - vanadate , chemistry , permeability (electromagnetism) , biophysics , analytical chemistry (journal) , nuclear chemistry , membrane , inorganic chemistry , biochemistry , chromatography , biology
We found that vanadate‐induced 45 Ca 2+ uptake by red cells is maximal at 25°C. At this temperature, the Ca i ‐induced increase of the K + permeability (the Gardos effect) shows a lag (up to 8 min) which is not observed at 37°C. This cannot be explained by the lack of availability of Ca 2+ for the Ca 2+ ‐activated K + channel, and suggests that its activation by Ca 2+ is mediated by a temperature‐dependent mechanism which remains unknown so far. The lag is not observed when the Gárdos effect was initiated by propranolol. This shows that the putative temperature‐dependent step is different from chloride transport.