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Human tRNA Glu genes: their copy number and organisation
Author(s) -
Gonos Efstathios S.,
Goddard John P.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80527-p
Subject(s) - gene , genetics , southern blot , dna , biology , genomic dna , restriction enzyme , microbiology and biotechnology , transfer rna , dna sequencing , sequence (biology) , rna
The tRNA Glu gene copy number, determined by genomic blot analysis of human plaeental DNA, is approximately thirteen. These studies, using several probes and DNA digested with several restriction enzymes singly or in combination, show that most of these tRNA Glu genes are flanked by DNA of very similar sequence for at least 5 kb. This conclusion is supported by the close similarity of the restriction maps of two λ Charon‐4A recombinants of human genomic DNA containing two different tRNA glu genes.