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Proteolytic activation of a bovine brain protein with phosphatidylinositol transfer activity
Author(s) -
van den Akker Willem M.R.,
Westerman Jan,
Gadella Theodorus W.J.,
Wirtz Karel W.A.,
Snoek Gerry T.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80523-l
Subject(s) - chemistry , phosphatidylinositol , microbiology and biotechnology , biochemistry , biology , signal transduction
We have purified a 38 kDa protein from bovine brain which is cross‐reactive with an affinity purified antibody against the 35 kDa phosphatidylinositol transfer protein from the same source. Controlled trypsinization of the 38 kDa protein yielded an immunoreactive protein of 35 kDa which displayed a 6‐fold increase in phosphatidylinositol transfer activity and a 10‐fold higher affinity for this phospholipid. The possibility that the 38 kDa protein is a precursor of the phosphatidylinositol transfer protein is discussed.