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Irreversible inhibition of D‐3‐aminoisobutyrate‐pyruvate aminotransferase by gabaculine
Author(s) -
Kaneko Masae,
Fujimoto Shigeko,
Kikugawa Mariko,
Tamaki Nanaya
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80521-j
Subject(s) - biochemistry , chemistry , enzyme , alanine , substrate (aquarium) , biology , amino acid , ecology
Gabaculine, 5‐amino‐l,3‐cyclohexadienylcarboxylate, is an analogue of GABA and a potent irreversible inhibitor of GABA aminotransferase. However, D‐3‐aminoisobutyrate‐pyruvate aminotransferase for which GABA was neither a substrate nor an inhibitor was also inactivated by gabaculine. The K i for D‐3‐aminoisobutyrate‐pyruvate aminotransferase was 8.3 × 10 −6 M, and the K cat fot its turnover was 0.31 min −1 at 25°C. β‐Alanine protected the enzyme from inactivation by gabaculine, but GABA did so to much a lesser extent.