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Galanin evokes a cytosolic calcium bursting mode and hormone release in GH3/B6 pituitary cells
Author(s) -
Guérineau Nathalie,
Drouhault Robert,
Corcuff Jean-Benoît,
Vacher Anne-Marie,
Vilayleck Nidaravone,
Mollard Patrice
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80520-s
Subject(s) - galanin , bursting , endocrinology , medicine , chemistry , anterior pituitary , cytosol , channel blocker , prolactin , calcium , intracellular , neuropeptide , biology , hormone , receptor , biochemistry , neuroscience , enzyme
The effects of galanin on secretion and cytosolic free Ca 2+ concentration ([Ca 2+ i ) have been studied in GH3/B6 pituitary cells. Prolactin (PRL) and growth hormone (GH) release was measured in column Perifusion experiments; [Ca 2+ ] i was monitored in single cells by dual emission microspectrofluorimetry using indo‐1 as intracellular Ca 2+ probe. Galanin (0.1–1 nM) caused PRL and GH release coincident with a modest rise in [Ca 2+ ] i The increase in [Ca 2+ ] i comprises the establishment of characteristic long‐lasting bursts of [Ca 2+ ] i transients. Galanin acts on Ca 2+ entry through voltage‐gated Ca 2+ channels since there was no response to the peptide when Cd 2+ ‐ a Ca 2+ channel blocker ‐ was added to the bath solution. The stimulation of bursting activity by galanin may provide a fine Ca 2+ ‐signalling mechanism which maximally stimulates hormone release while avoiding refractory periods.