Premium
Chloroplast ribosomal protein L32 is encoded in the chloroplast genome
Author(s) -
Yokoi Fumiaki,
Vassileva Anelia,
Hayashida Nobuaki,
Torazawa Keita,
Wakasugi Tatsuya,
Sugiura Masahiro
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80514-j
Subject(s) - chloroplast , ribosomal protein , chloroplast dna , biology , genome , protein subunit , ribosomal rna , genetics , gene , homology (biology) , ribosome , microbiology and biotechnology , rna
The 50 S subunit of chloroplast ribosomes was prepared from tobacco leaves. The proteins were fractionated and the N‐terminal amino acid sequence of a 14 kDa protein was determined. This sequence matches the N‐terminal sequence deduced from ORF55 located between ndhF and trnL on the small single‐copy region of tobacco chloroplast DNA. The deduced protein shows homology to E. coli and B. stearothermophilus L32 proteins, and it has been named as CL32 and ORF55 as rpl32 . The tobacco chloroplast genome therefore contains 21 different ribosomal protein genes.