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Reversible ADP‐ribosylation of the 78 kDa glucose‐regulated protein
Author(s) -
Leno Gregory H.,
Ledford Barry E.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80499-9
Subject(s) - adp ribosylation , adp ribosylation factor , chemistry , biochemistry , microbiology and biotechnology , biology , enzyme , nad+ kinase , golgi apparatus , cell
Starvation of Mouse hepatoma cells for essential amino acids or glucose results in the mono‐ADP‐ribosylation of the 78 kDa glucose‐regulated protein, GRP78. Here we show that the ADP‐ribosylated and non‐ADP‐ribosylated forms of GRP78 are interconvertible during tryptophan starvation and refeeding. In addition, the ADP‐ribosylation of GRP78 was shown to be reversible even during nutritional stress. The overexpressed pool of non‐ADP‐ribosylated GRP78 synthesized during tunicamycin treatment was available for ADP‐ribosylation during subsequent amino acid starvation, especially in the absence of tunicamycin. The reversible ADP‐ribosylation of GRP78 could be part of a metabolic control mechanism in operation during nutritional stress.