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Gap junctional permeability is affected by cell volume changes and modulates volume regulation
Author(s) -
Ngezahayo Anaclet,
Kolb Hans-Albert
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80493-3
Subject(s) - permeability (electromagnetism) , volume (thermodynamics) , chemistry , microbiology and biotechnology , biophysics , biology , biochemistry , membrane , physics , thermodynamics
Isolated pancreatic acinar cell pairs became electrically uncoupled by exposure to a mild hypotonic shock. Reduction of bath osmolarity caused a delayed closure of gap junctional channels in the minute range. Dialysis of cell pairs by GTP[S] in the double whole‐cell patch‐clamp mode shortened the latency and shifted the hypotonically induced electrical uncoupling to lower osmolarity changes. Cellular treatment with cytochalasin B catalyzed electrical uncoupling by a hypotonic shock. In all cases, electrical uncoupling could be blocked completely by the protein kinase C (PKC) inhibitor polymyxin B. These results provide the first evidence suggesting that changes of cell volume and gap junctional permeability are correlated and that a G‐protein dependent mechanism is involved. Evidence is presented that gap juntional coupling modulates volume regulation.