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Carboxyatractylate‐sensitive uncoupling in liver mitochondria from ground squirrels during hibernation and arousal
Author(s) -
Brustovetsky N.N.,
Amerkanov Z.G.,
Yegorova M.E.,
Mokhova E.N.,
Skulachev V.P.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80481-w
Subject(s) - oligomycin , mitochondrion , hibernation (computing) , oxidative phosphorylation , respiration , membrane potential , chemistry , atp synthase , biochemistry , biology , medicine , anatomy , atpase , enzyme , state (computer science) , algorithm , computer science
Energy coupling parameters of liver mitochondria from hibernating and arousing ground squirrels have been studied. In the oligomycin‐treated mitochondria, carboxyatractylate, an inhibitor of the ATP/ADP‐antiporter, is shown to decrease the respiration rate, to increase the membrane potential and to lower the rate of the membrane‐potential discharge after the addition of cyanide to liver mitochondria from hibernating and arousing animals. BSA effectively substitutes for carboxyactactylate so that carboxyactactylate, added after BSA, has no effect. In mitochondria from hibernating animals, the maximal respiration rate in the presence of DNP and the rate of the membrane potential discharge in its absence are much lower than in those from arousing animals. It has been concluded that upon arousal of the animals from hibernation, the uncouling of oxidative phosphorylation, mediated by free fatty acids and ATP/ADP‐antiporter, parallels the respiratory chain activation.