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Transient versus steady state NOE in paramagnetic molecules Cu 2 Co 2 SOD as an example
Author(s) -
Banci Lucia,
Bertini Ivano,
Luchinat Claudio,
Piccioli Mario
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80477-z
Subject(s) - spins , chemistry , steady state (chemistry) , paramagnetism , hyperfine structure , transient (computer programming) , molecule , atomic physics , nuclear magnetic resonance , physics , quantum mechanics , condensed matter physics , computer science , organic chemistry , operating system
Truncated, steady state and transient NOE experiments have been performed on bovine Cu 2 Co 2 Superoxide dismutase. The effectiveness of the different NOE experiments in the general case of paramagnetic macromolecules is discussed. It is concluded that steady state NOEs give superior results. The validity of the two spins approximation is discussed, and NOE values for a fully coupled set of nuclei have been calculated. Transient NOE experiments, when properly performed, confirm the previous assignment of the hyperfine shifted signals in Cu 2 Co 2 SOD based on steady state NOE measurements [(1989) Inorg. Chem. 28, 4650] and eliminate any further reason of controversy on an important issue as the assignment of the 1 H NMR signals of protons of metal‐coordinated imidazoles.