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Biosynthesis of cyclic 2,3‐diphosphoglycerate
Author(s) -
Lehmacher Anselm,
Vogt Anne-Barbara,
Hensel Reinhard
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80456-s
Subject(s) - diphosphoglycerate , biosynthesis , phosphoglycerate kinase , chemistry , enzyme , biochemistry , intramolecular force , phosphorylation , stereochemistry , hemoglobin
Starting from 2‐phosphoglycerate the biosynthesis of cDPG comprises two steps: (i) the phosphorylation of 2‐phosphoglycerate to 2,3‐diphosphoglycerate and (ii) the intramolecular cyclization to cyclic 2,3‐diphosphoglycerate. The involved enzymes, 2‐phosphoglycerate kinase and cyclic 2,3diphosphoglycerate synthetase, were purified form Methanothermus fervidus . Their molecular and catalytic properties were characterized.