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Protein TAU variants present in paired helical filaments (PHFs) of alzheimer brains
Author(s) -
Gache Y.,
Ricolfi F.,
Guilleminot J.,
Theiss G.,
Nunez J.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80450-w
Subject(s) - polyclonal antibodies , tau protein , antiserum , alzheimer's disease , chemistry , microbiology and biotechnology , sequence (biology) , biochemistry , antibody , biology , pathology , genetics , medicine , disease
Polyclonal anti‐TAU antisera directed against native Tau protein and the NH 2 ‐terminal side of the mouse TAU sequence were used to determine the nature of the TAU variants present in Alzheimer brains and in PHFs. These antibodies labelled specifically neuroflbrillary tangles and plaque neuntes in Alzheimer brains. On immunoblots of PHF extracts, two entities of 69 and 130 kDa were identified. These TAU‐related species were absent from control brains. Protein immunoblot of total Alzheimer and control supernatants were shown to contain the same 4–5 TAU variants but none of the 69 and 130 TAU‐related entities found in PHFs. These data suggest that specific TAU species are present in PHFs.