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The RNA component of RNase P in Schizosaccharomyces species
Author(s) -
Zimmerly Steven,
Gamulin Vera,
Burkard Ulrike,
Söll Dieter
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80403-6
Subject(s) - schizosaccharomyces pombe , rna , rnase p , gene , biology , non coding rna , genetics , schizosaccharomyces , rna silencing , signal recognition particle rna , microbiology and biotechnology , rna interference , saccharomyces cerevisiae
In the fission yeast Schizosaccharomyces pombe , the enzyme RNAse P copurifies with two RNAs, K1‐ and K2‐RNA, which are identical except for their termini [1] and which are encoded by a single gene [2]. We have undertaken the cloning of the K‐RNA genes in related organisms in order to gain comparative structural information. Because the K‐RNA sequence is poorly conserved across species, we have cloned the K‐RNA genes in the Schizosaccharomyces species S. malidevorans, S. japonicus, S. versatilis , and S. octosporus . Of the 4 species, only S. octosporus contains a K‐RNA gene different from that in S. pombe ; the gene diverges by 20%. Based on the two sequences, nuclease protection data and computer analysis, we have proposed a secondary structure model for the K‐RNA. Northern analysis shows the K‐RNA genes in all four Schizosaccharomyces species to be expressed as two RNAs, as in S. pombe .