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Primary structure and functional expression from cDN A of the cardiac ryanodine receptor/calcium release channel
Author(s) -
Nakai Junichi,
Imagawa Toshiaki,
Hakamata Yasuhiro,
Shigekawa Munekazu,
Takeshima Hiroshi,
Numa Shosaku
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80399-4
Subject(s) - ryanodine receptor , ryanodine receptor 2 , complementary dna , biology , microbiology and biotechnology , cardiac muscle , biochemistry , endoplasmic reticulum , endocrinology , gene
The sequence of 4968 (or 4976 with an insertion) amino acids composing the ryanodine receptor from rabbit cardiac sarcoplasmic reticulum has been deduced by cloning and sequencing the cDNA. This protein is homologous in amino acid sequence and shares characteristic structural features with the skeletal muscle ryanodine receptor. Xenopus oocytes injected with mRNA derived from the cardiac ryanodine receptor cDNA exhibit Ca 2+ ‐dependent Cl − current in response to caffeine, which indicates the formation of functional calcium release channels. RNA blot hybridization analysis with a probe specific for the cardiac ryanodine receptor mRNA shows that the stomach and brain contain a hybridizable RNA species with a size similar to that of the cardiac mRNA. This result, in conjunction with cloning and analysis of partial cDNA sequences, suggests that the brain contains a cardiac type of ryanodine receptor mRNA.