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Membrane lateral pressure as a modulator of glycerol‐3‐phosphate dehydrogenase activity
Author(s) -
Amler Evžen,
Jasińska Renata,
Drahota Zdeněk,
Zborowski Józef
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80398-3
Subject(s) - dehydrogenase , chemistry , phosphate , membrane , glycerol , phosphatidylserine , biochemistry , enzyme , biophysics , kinetics , phospholipid , biology , physics , quantum mechanics
Michaelis‐Menten kinetics of glycerol‐3‐phosphate dehydrogenase activity in proteoliposomes from brown adipose tissue mitochondria with exogenously added phospholipids or cholesterol was measured. It was shown that changes in membrane lipid composition affected the membrane lateral pressure and therefore modulated the enzyme activity, namely V max value. Contrarily, changes in surface charge caused by minute amounts of phosphatidylserine or charged organic substances influenced only the apparent K m value. The role of bulk phospholipids in regulation of glycerol‐3‐phosphate dehydrogenase is discussed.