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Detection of a large cation‐selective channel in nuclear envelopes of avian erythrocytes
Author(s) -
Matzke A.J.M.,
Weiger T.M.,
Matzke M.A.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80397-2
Subject(s) - ion channel , inner membrane , chemistry , biophysics , membrane , envelope (radar) , nuclear membrane , biology , biochemistry , telecommunications , radar , receptor , computer science
To determine whether the nuclear envelope of eukaryotic cells has the capability to regulate ion fluxes, we have used the patch‐clamp technique to detect ion channels in this membrane system. Since possible sites for ion channels in the nuclear envelope include not only the nuclear pores, but also both the inner and outer nuclear membranes, we have patched giant liposomes composed of phosphatidylcholine and nuclear envelope fragments isolated from mature avian erythrocytes. A large, cation‐selective channel with a maximum conductance of approximately 800 pS in symmetrical 100 mM KCl was detected. This channel is a possible candidate for a nuclear pore.