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Release of the component of Streptococcus faecalis Na + ‐ATPase from the membranes
Author(s) -
Kakinuma Yoshimi,
Igarashi Kazuei
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80382-s
Subject(s) - ethylenediaminetetraacetic acid , membrane , chemistry , trypsin , atpase , incubation , f atpase , chromatography , streptococcaceae , biochemistry , enzyme , streptococcus , bacteria , chelation , biology , inorganic chemistry , antibiotics , thylakoid , chloroplast , gene , genetics
The Na + ‐stimulated ATPase activity of Streptococcus faecalis was lost by washing the membranes with ethylenediaminetetraacetic acid (EDTA). ATPase activities of both the EDTA extract and the stripped membranes did not show any stimulation by Na + ions. However, the Na + ‐stimulated ATPase was readily reconstituted by an incubation of these fractions combined. It was only reconstituted from the fractions prepared under the condition that the Na + ‐ATPase is amplified, and not from those boiled or digested by trypsin. Thus, the component of Na + ‐ATPase of this organism is capable of being released from the membranes.