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Newly identified type of β actin reduces invasiveness of mouse B16‐melanoma
Author(s) -
Sadano Hiroyuki,
Taniguchi Shun'ichiro,
Baba Tsuneo
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80363-n
Subject(s) - actin , melanoma , transfection , cytoskeleton , microbiology and biotechnology , cell culture , biology , phenotype , stress fiber , actin cytoskeleton , cancer research , cell , gene , genetics
Low metastatic parent B16 melanoma and isolated B16‐F1 cell lines have a third actin designated as βm(A x :previously). ßm actin is scantily or not at all detected in highly metastatic cell lines, such as B16‐F10 and BL6. To directly assess the physiological role of ßm in phenotypic changes of B16 melanoma, we transfected expression plasmids of βm into B16‐F10 cells. The actin expressed in the transfectants is located largely in cytoskeletal fractios. The transfectants exhibited a larger number of stress fibers and a lower invasiveness than did the recipient cells. Thus, βm actin plays an important role in the organization of actin stress fibers, the result being a decrease in invasiveness of B16 melanoma.