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Monoclonal antibody to desmin purified from cow Purkinje fibers reveals a cell‐type specific determinant
Author(s) -
Virtanen I.,
Närvänen O.,
Thornell L.-E.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80317-c
Subject(s) - desmin , monoclonal antibody , antibody , chemistry , microbiology and biotechnology , biology , immunology , immunohistochemistry , vimentin
We have raised monoclonal antibodies (Mab) to the M r 55000 desmin polypeptide, electrophoretically purified from cytoskeletal preparations of isolated bovine heart Purkinje fibers. One of the Mabs, 39AB6, revealed desmin only in cow Purkinje fibers and did not react with desmins from other muscle cells, including ventricular cardiac muscle, striated muscle and smooth muscle, as revealed by both immunoblotting and immunocytochemistry. Desphosphorylation of electrophoretically separated polypeptides on nitrocellulose with alkaline phosphatase did not affect the binding of the Mab. The present results show that there are cell‐type specific antigenic determinants in intermediate proteins of the desmin type.