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High mobility group proteins 1 and 2 bind preferentially to brominated poly(dG‐dC)·poly(dG‐dC) in the Z‐DNA conformation but not to other types of Z‐DNA
Author(s) -
Christen T.,
Bischoff M.,
Hobi R.,
Kuenzle C.C.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80308-6
Subject(s) - high mobility group , dna , homology (biology) , chemistry , dna binding protein , microbiology and biotechnology , z dna , biology , biochemistry , gene , transcription factor
Three proteins from bull testis, previously thought to be Z‐DNA‐binding proteins but recently found to recognize brominated poly(dG‐dC)·poly(dG‐dC) by criteria different from the Z‐conformation, were partially sequenced. Of these, the 31 kDa protein was identified as a member of the high mobility group 2 protein family, and the 33 kDa protein as a member of the high mobility group 1 protein family. Both proteins had molecular weights approximately 30% higher than expected, indicating considerable posttranslational modification. In contrast, the 58 kDa protein remained unidentified for lack of any significant homology with known protein sequences.