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Essential role of ferrous iron in cyanide‐resistant respiration in Hansenula anomala
Author(s) -
Minagawa Nobuko,
Sakajo Shigeru,
Komiyama Tadazumi,
Yoshimoto Akio
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80302-y
Subject(s) - cyanide , respiration , antimycin a , cycloheximide , ferrous , chemistry , cellular respiration , biochemistry , phenanthroline , nuclear chemistry , biology , protein biosynthesis , mitochondrion , inorganic chemistry , organic chemistry , botany
Antimycin A‐dependent induction of cyanide‐resistant respiration in Hansenula anomala was completely blocked by o ‐phenanthroline, α,α'‐dipyridyl. or 8‐hydroxyquinoline. Pulse‐labeling of the cells with [ 35 S] methionine in the presence of both antimycin A and o ‐phenanthroline indicated that the 36‐kDa protein previously reported to be involved in cyanide‐resistant respiration [(1989) J. Biochem. 105, 864‐866] was formed in mitochondria even under these conditions. The addition of Fe 2+ , but not Fe 3+ , ions to these cells in the presence of cycloheximide resulted in the rapid expression of cyanide‐resistant respiration activity. These results suggest that in the presence of both antimycin A and o ‐phenanthroline an inactive form of the 36‐kDa protein was formed and Fe 2+ ions converted it to the active form. It is also likely that Fe 2+ ions are involved in the reaction mechanism of cyanide‐resistant respiration.