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Effect of ATP on the activity of bovine heart mitochondrial b‐c 1 complex
Author(s) -
Lorusso Michele,
Cocco Tiziana,
Minuto Michele,
Papa Sergio
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80299-x
Subject(s) - egta , enzyme , chemistry , antimycin a , biochemistry , chelation , steady state (chemistry) , mitochondrion , stereochemistry , calcium , inorganic chemistry , organic chemistry
The effect of ATP on the reductase activity of purified bovine heart b‐c 1 complex was studied. ATP stimulates the steady‐state activity and the antimycin‐insensitive pre‐steady‐state reduction of b and c 1 cytochromes, also causing changes of kinetic properties of the enzyme. There is no absolute specificity for ATP since other polyvalent anions such as EDTA and EGTA produce similar effects in the micromolar range. It is proposed that ATP stimulates the activity of the b‐c 1 complex, chelating inhibitory cation(s), exerting a modulatory action on the enzyme.