Premium
Distribution of inositol 1,4,5‐trisphosphate receptor mRNA in mouse tissues
Author(s) -
Furuichi Teiichi,
Shiota Chiyo,
Mikoshiba Katsuhiko
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80294-s
Subject(s) - oviduct , northern blot , biology , in situ hybridization , messenger rna , uterus , receptor , ovary , endocrinology , medicine , second messenger system , kidney , microbiology and biotechnology , biochemistry , gene
Northern blot analysis demonstrated that the concentration of inositol 1,4,5‐trisphosphate (IP 3 ) receptor mRNA was greatest in cerebellar tissue. Moderate amounts of IP 3 mRNA were present in brain tissue without cerebellum and tissue of the thymus, heart, lung, liver, spleen, kidney, and uterus. Small amounts of IP 3 receptor mRNA were observed in skeletal muscle and testicular tissue. Regional distribution of IP 3 mRNA in various tissues was also examined by in situ hybridization. A considerable amount of IP 3 receptor mRNA was located in smooth muscle cells, such as those of the arteries, bronchioles, oviduct and uterus. In addition, secondary oocytes surrounded by Graafian follicles in the ovary were found to have large amounts of IP 3 receptor mRNA. The present studies suggest a functional importance of the IP 3 second‐messenger system in these cell types.