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Amino acid sequence of pheromone‐inducible surface protein in Enterococcus faecalis , that is encoded on the conjugative plasmid pPD1
Author(s) -
Nakayama Jiro,
Nagasawa Hiromich,
Isogai Akira,
Clewell Don B.,
Suzuki Akinori
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80293-r
Subject(s) - enterococcus faecalis , plasmid , enterococcus , biology , peptide sequence , sequence (biology) , pheromone , microbiology and biotechnology , genetics , bacteria , gene , escherichia coli
The major pheromone‐inducible protein, PD78, believed to contribute to bacterial conjugation, was purified from Enterococcus (formerly Streptococcus) faecalis cells containing the plasmid pPD1. A cloned Eco RI‐ Bg /II 3.6‐kbp fragment of the plasmid pAM351 (pPD1::Tn 916 ) contained an open reading frame corresponding to 467 amino acid residues representing PD78. In a central region of the deduced protein, there is a repeated sequence of X‐X‐Pro that is repeated 15 times. This is analogous to the Gln‐Gln‐Pro repeat in the C‐terminal region of TraD product encoded on the R 100 plasmid in Escherichia coli .