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ATP‐dependent regulation of phospholipase C in permeabilized 3T3 cells
Author(s) -
Higashi Kyoichiro,
Ogawara Hiroshi
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80285-q
Subject(s) - 3t3 cells , chemistry , microbiology and biotechnology , biochemistry , biology , gene , transfection
Regulation of phospholipase C (PLC) coupled with a G‐protein was studied with Swiss 3T3 cells permeabilized by digitonin. In permeabilized cells, activation ofphospholipase C required millimolar concentrations of ATP in addition to a G‐protein activator, AlF 4 − or nonhydrolysable GTP analogues. To determine the mechanism of the action of ATP, we examined the effects of ATP analogues. ATPγS directly activated phospholipase C in the presence or absence ofAlF 4 − . On the other hand, neither βγ‐methylene ATP nor adenyl‐5'‐yl imidodiphosphate nor ADPβS could support the AlF 4 − ‐dependent activation of phospholipase C. The action of ATPγS was not through the substrate supply for phospholipase C, because ATPγS did not augment the levels of PIP 2 or PIP in permeabilized cells. These results suggested the significance of the γ‐phosphate group of ATP and/or phosphorylation by ATP in the activation of phospholipase C by a putative G‐protein.