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RGD peptides may only temporarily inhibit cell adhesion to fibronectin
Author(s) -
Ylänne Jari
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80283-o
Subject(s) - fibronectin , chemistry , cell adhesion , adhesion , microbiology and biotechnology , cell , biochemistry , biology , organic chemistry
When human fibroblasts were cultured on fibronectin for 4 h in the presence of 0.5of the GRGDSP peptide derived from the fibronectin cell‐binding site, they adhered and spread normally and organized talin and integrin α 5 and β 1 subunits into focal adhesions. When the adherent cells were quantitated as a function of time, submaximal peptide concentrations were found to delay cell adhesion on fibronectin, but they had no effect on the maximum. When the cells were plated on vitronectin, however, even relatively low peptide concentrations lowered the maximal amount of cells adhering and abolished cell spreading. The results suggest a different mechanism for cell adhesion on fibronectin and vitronectin.