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Electron microscopy of native and artificial methylreductase high‐molecular‐weight complexes in strain Gö 1 and Methanococcus voltae
Author(s) -
Hoppert M.,
Mayer F.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80281-m
Subject(s) - methanococcus , strain (injury) , bacteria , vesicle , chemistry , electron microscope , cytoplasm , membrane , biochemistry , biophysics , morphology (biology) , stereochemistry , biology , escherichia coli , genetics , physics , anatomy , optics , gene
The preparation of inside‐out vesicles from methanogenic bacteria with protein cell walls was improved with regard to the preservation of structure and localization of membrane‐bound proteins. Complexes similar to the methanoreductosome in the methanogenic bacterium Gö 1 [1] were also found attached to the inner aspect of the cytoplasmic membrane of Methanococcus voltae . Methanoreductosomes were purified from crude extracts of Gö 1‐cells by affinity chromatography. Under specific conditions at high protein concentrations methyl‐CoM‐methylreductase molecules isolated from Gö 1‐cells could be reassociated to spherical complexes of various sizes, with an appearance similar to that of methanoreductosomes isolated from strain Gö 1.