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ESR spin trapping and NMR spectroscopy of the same heart shows correlation between energy depression and radical formation during postischemic reperfusion
Author(s) -
Blasig I.E.,
Steinschneider A.Y.,
Lakomkin V.L.,
Ledenev A.N.,
Korchazhkina O.V.,
Ruuge E.K.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80280-v
Subject(s) - trapping , spectroscopy , chemistry , nuclear magnetic resonance spectroscopy , spin (aerodynamics) , spin trapping , nuclear magnetic resonance , materials science , electron paramagnetic resonance , physics , stereochemistry , biology , ecology , quantum mechanics , thermodynamics
The relevance of radical formation in disturbances of energy metabolism in the postischemic heart is not clear. This study provides the first evidence of a significant correlation between the amount of oxy‐radicals trapped in the effluent of isolated hearts upon reperfusion and the decreased myocardial content of phosphocreatine and ATP. This suggests that the loss of high‐energy compounds might contribute to oxy‐radical production during reperfusion. The application of ESR spin trapping and of NMR technique to the same heart is a new approach to investigate the pathobiochemical relevance of free radicals for the heart muscle.