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Modulation of vertebrate brain Na + and K + channels by subtypes of protein kinase C
Author(s) -
Lotan Ilana,
Dascal Nathan,
Naor Zvi,
Boton Rony
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80279-r
Subject(s) - protein kinase c , xenopus , chemistry , intracellular , phorbol , microbiology and biotechnology , biophysics , kainate receptor , protein kinase a , endocrinology , biology , enzyme , medicine , biochemistry , receptor , glutamate receptor , ampa receptor , gene
Effects of purified subtypes I, II and III of protein kinase C (PKC) on voltage‐dependent transient K + (A) and Na + channels were studied in Xenopus oocytes injected with chick brain RNA. The experiments were performed in the constant presence of 10 nM β‐phorbol 12‐myristate‐13‐acetate (PMA). Intracellular injection of subtype I (τ) reduced the A‐current ( I A ), with no effect on Na + current ( I Na ). PKC subtype II (β 1 , + β 2 ) and III (α) reduced both currents. PKC did not affect the response to kainate. Inactivated (heated) or unactivated (injected in the absence of PMA) enzyme and vehicle alone had no effect. Our results strongly suggest that I Na , and I A in vertebrate neurons are modulated by PKC; all PKC subtypes exert a similar effect on the A‐channel while only subtypes II and III modulate the Na + channel.