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A novel combination of prosthetic groups in a fungal laccase; PQQ and two copper atoms
Author(s) -
Karhunen Eija,
Niku-Paavola Marja-L.,
Viikari Liisa,
Haltia Tuomas,
van der Meer Robert A.,
Duine Johannis A.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80273-l
Subject(s) - laccase , coniferyl alcohol , lignin , chemistry , oxidoreductase , molecule , copper , cofactor , electron paramagnetic resonance , oxygen , alcohol , photochemistry , organic chemistry , enzyme , stereochemistry , nuclear magnetic resonance , physics
Extracellular laccase (benzenediol:oxygen oxidoreductase EC 1.10.3.2) from the lignin‐degrading fungus, Phlebia radiata , was shown to contain a novel combination of electron carriers as its prosthetic groups. In addition to two copper atoms per enzyme molecule, one molecule of PQQ was included as a cofactor. The EPR spectrum exhibits features of type 1 and type 2 copper atoms. In the enzymatic reaction 4 molecules of lignin model compound, coniferyl alcohol, are oxidized per molecule of oxygen reduced to water. During the reaction coniferyl alcohol is transformed to dilignols.