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2D COSY 1 H NMR: a new tool for studying in situ brain metabolism in the living animal
Author(s) -
Barrére Bertrand,
Peres Michel,
Gillet Brigitte,
Mergui Simone,
Beloeil Jean-Claude,
Seylaz Jacques
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80247-g
Subject(s) - phosphocreatine , taurine , creatine , glutamine , choline , in situ , alanine , chemistry , glutamate receptor , nuclear magnetic resonance spectroscopy , biochemistry , betaine , metabolism , amino acid , biology , stereochemistry , energy metabolism , endocrinology , receptor , organic chemistry
2D COSY 1 H NMR with surface coil has been used to resolve and assign cerebral metabolites which had previously been detected but could not be resolved or assigned in situ in the living animal by conventional ID 1 H NMR. A wide range of cerebral metabolites, including alanine, N ‐acetyl aspartate, aspartate, choline derivatives, creatine/phosphocreatine pool, GABA, glucose, glutamate/glutamine pool, inositol, lactate and taurine were simultaneously resolved and assigned in situ in the whole animal using the 2D COSY correlation graphs. Global irreversible ischemia caused the appearance and the disappearance of cross‐peaks in the 2D COSY 1 H NMR map, corresponding to increases in alanine, GABA and lactate and glucose depletion.