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Microtubule translocation caused by three subspecies of inner‐arm dynein from Chlamydomonas flagella
Author(s) -
Kagami Osamu,
Takada Saeko,
Kamiya Ritsu
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80243-c
Subject(s) - chlamydomonas , dynein , flagellum , microtubule , microbiology and biotechnology , dynein atpase , biology , biophysics , chemistry , mutant , biochemistry , gene
To help understand the function of inner‐arm dynein in nagellar motility, dynein samples from an outer arm‐missing mutant of Chlamydomonas (odal) were examined for the ability to translocate microtubules in vitro. High‐salt extract ofaxonemes containing inner‐arm dynein was separated by ion‐exchange chromatography into 7 peak fractions with ATPase activities. Of these, three fractions containing different sets of dynein heavy chains translocated microtubules. The maximal velocities were all between 3 and 5, which were comparable to the microtubule sliding rate in disintegrating oda axonemes.